Composite

Part:BBa_K346035:Design

Designed by: Junyi Jiao   Group: iGEM10_Peking   (2010-10-14)


Ptet-T7polymerase+Hg(II) Bioabsorption Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 317
    Illegal BamHI site found at 1148
    Illegal BamHI site found at 2111
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal AgeI site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Only in the DH 5alpha strain can the part expresses in a constotuitive way.


Source

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References